1
We obtained archival paraffin-embedded tissues from patients with invasive breast cancer and varying axillary lymph node involvement.
2
Detection of such mutations in archival paraffin-embedded biopsies is usually hampered by an abundance of surrounding normal cells.
3
Pathology slides and archival paraffin blocks were retrieved for confirmation of the original diagnosis, study of histopathologic features, and measurement of p21 content.
4
As both diagnostic techniques can be applied to widely available archival paraffin-embedded tissues, the usefulness of the strategy is largely implicated in routine pathology laboratories.
5
Our results indicate that dHPLC and HRM are techniques that can be reliably used for the detection of the BRAFV600E mutation in archival paraffin-embedded tissues.
6
Methods: Archival paraffin-embedded tumor specimens from patients undergoing CRT between 1995 and 2001 were analyzed.