1
When we send semen out to our clients the specimens are cryopreserved in screw-top vials.
2
In Experiment 2, cooled-transported semen was centrifuged at room temperature and cryopreserved in three semen freezing extenders.
3
Semen was collected from five rams and cryopreserved in a Tris-based extender supplemented with glycerol and egg yolk as the main cryoprotectants.
4
After dilution, semen samples were cryopreserved in 0.5 mL straws for 10 days in a liquid nitrogen tank.