We have no meanings for "using transient" in our records yet.
Methods: We develop a method to expand wild-type and dystrophic fresh isolated satellite cells using transient expression of Pax3.
This was originally deduced from their strong fluorescence solvatochromism and later visualized in real time using transient infrared spectroscopy.
Diagnostic yield was 90% using transient, but less than 20% using steady-state stimuli.
The results suggest that the effect of DNA size should be taken into consideration for evaluation of transcriptional elements using transient transfection analysis.
Using transient expression of cDNAs in COS cells, we studied the interaction of BiP-tag with several membrane proteins.
Using transient transfection systems, we demonstrated that Smad3 specifically represses transcriptional activation mediated by AR on two natural androgen-responsive promoters.
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