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1
Osteoblast-like cells were cultured from human foetal bone chips
grown
to
confluence
and used at first subculture.
2
Primary cultured cortical astrocytes were prepared from male and female rat pups separately and
grown
to
confluence
in estrogen-free media.
3
For cytotoxicity assays ARPE19 cells were
grown
to
confluence
and then cultured in a serum-deficient medium to ensure a static milieu.
4
Almost completely homogenous gastric mucous epithelial cells of guinea pigs were
grown
to
confluence
in the presence of 10% fetal calf serum (FCS).