Mineral deposition was monitored using alizarinred as a vital stain for calcium.
2
Mineralized matrix formation was visualized using alizarinred S staining.
3
Approach and results: Arterial calcium accumulation was evaluated using alizarinred stain and atomic absorption spectrometry.
4
The results of alizarinred staining and ALP detection showed that SpA treatment suppressed the osteogenic differentiation of hBMSCs.
5
Cell counting kit-8 activity was examined to test cell proliferation, and alkaline phosphatase assays and alizarinred S staining were conducted to examine differentiation.
6
Alizarinred staining and ALP activity detection were carried out.
7
Bone formation was assessed by AlizarinRed extraction.
8
AlizarinRed S and Oil Red O staining were used to identify osteoblast and adipocyte differentiation, respectively.
9
Only FK-506 was able to enhance osteocalcin transcripts and AlizarinRed staining, both late markers for differentiation.
10
Flow cytometry, Oil Red O stain and AlizarinRed stain were used to identify the characteristics of HF-MSCs.
11
Alkaline phosphatase (ALP) staining and AlizarinRed staining were used for pathological analysis of the cells.
12
Alizarinred S-positive nodules were first observed on day 14 and then increased in number during the entire culture period.
13
Calcification was verified using Calcein and AlizarinRed S staining or Calcium assays, and molecular analyses showed enhanced expression of calcification-associated genes.
14
Aortic calcification was assessed in 28-day-old mice by measuring the uptake of a fluorescent bisphosphonate probe and by staining tissue sections with Alizarinred.