1
We have developed a rapid and general technique for purification of a protein encoded by a cistron contained in a recombinant DNA clone.
2
We report the discovery of a short ORF embedded within the P3 cistron of the polyprotein but translated in the +2 reading-frame.
3
Our results suggest that a nonoverlapping cistron in the beta 2 receptor mRNA 5' leader region is translated and the resulting peptide inhibits receptor translation.
4
Members of the Paramyxoviridae family utilize a variety of different strategies to increase coding capacity within their P cistrons.
5
The techniques consist of tagging the initiator cistrons at the C-terminus with beta-galactosidase-encoding DNA of Escherichia coli in the correct translational phase.