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Results: Many of the unfertilized eggs were in metaphase and had relatively low corticalgranule densities indicative of corticalgranule loss.
2
To identify factors that influence the activity of separase in corticalgranule exocytosis and cytokinesis, we carried out a genetic suppressor screen.
3
Newly fertilized mutant embryos have defects in polar body extrusion, fail to undergo corticalgranule exocytosis, and subsequently fail to complete cytokinesis.
4
Conclusion: The observed changes in ZP biochemistry and corticalgranule quantitation demonstrate that failed fertilization is frequently associated with spontaneous cytoplasmic, but not nuclear, activation.
5
Objective: To determine the extent of biochemical changes in the zona pellucida (ZP) and corticalgranule release in eggs failing to fertilize in IVF.
6
The relationship of changes in the ZP and corticalgranules to infertility and in vitro culture requires further investigation.