Significats de direct mutagenesis en anglès

Improved efficiency and applicability have been achieved by direct mutagenesis and interserotype domain rearrangement.

How phosphorylation regulates the activity of the pln response regulators was also accessed by direct mutagenesis at their phosphorylation sites.

Computational docking studies combined with site- direct mutagenesis identified several key residues within the ligand-binding pocket of PXR that constitute points of interaction with amprenavir.

Site- directed mutagenesis revealed two N-glycosylation sites in a large putative extracellular loop.

Objective: To introduce a new technique for rapid construction of gene site- directed mutagenesis.

Conclusion: The method is effective and simple for construction of gene site- directed mutagenesis.

To directly assess functional effects, we conducted site- directed mutagenesis to generate mutant receptors.

Site- directed mutagenesis supports the conclusion that this is the active site.

Based on site- directed mutagenesis, functionally important residues of the DH domain were identified.

The mechanistic predictions from molecular dynamics simulations are validated using site- directed mutagenesis experiments.

Site- directed mutagenesis enabled a further analysis of the SUMO-target amino acids.

Directed mutagenesis of the protein reveals two highly conserved histidine residues required for function.

We have investigated residue 144 using aspartate and glutamine substitutions introduced by site- directed mutagenesis.

This was subsequently substantiated by the analysis of respective mutants constructed by site- directed mutagenesis.

Results: Two site- directed mutagenesis have been successfully constructed in the Parkin gene by this method.

We performed extended site- directed mutagenesis and measured binding rate constants through surface plasmon resonance analysis.