It is concluded that a hybridomacell line stably secretes specific mAb against UGP2.
2
A stable hybridomacell line was established.
3
The hybridomacell was adapted to serum-free medium and antibody was produced in a hollow fiber cell culture system (Technomouse).
4
An indirect ELISA method using 3T3-B7-H4 lysate as antigen was established to screen antibody-producing hybridomacell lines.
5
A library of hybridomacell lines has been established which produce monoclonal antibodies against antigens from the germinal vesicle ofXenopus laevis oocytes.
6
The hybridomacell line established from the fusion of CLL cell from two patients who were HTLV-I seropositive produced antibody directed against HTLV-I proteins.
7
Two hybridomacell lines secreting monoclonal antibodies against these peptides were established by cell fusion and 2 to 3 rounds of cell cloning.
8
In comparison, hybridomacells are only adsorbed to the surface and do not spread at all.
9
All the eight kinds of positive hybridomacells were mixed, cloned, screened by protein array, and definite dilution cloned.
10
Among these factors, the impact of micronutrients on the production of MAbs by mouse hybridomacells has not fully been explored.
11
Addition of CD8-OVA hybridomacells to plastic plates adsorbed with K(b)-OVA molecules resulted in a concentration-dependent decrease in cellular proliferation.
12
We cloned immunoglobulin G (IgG) variable domains from cryopreserved hybridomacells and input them into an integrated pipeline for expression and validation of functional R-mAbs.