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The ospC genotype was found to be different at each initial and second episode.

In individuals with ospC variation, at least seven ospC variants were recognized at each time point.

Nucleotide sequence information from different B. burgdorferi ospA genotypes was used to design primers homologous to different genotypes.

Using a genetic screen, we identified ospD1 as a gene whose product negatively controls expression of genes regulated by secretion activity.

After polymerase-chain-reaction amplification, ospC genotyping was performed by means of reverse line-blot analysis or DNA sequencing of the nearly full-length gene.