The occurrence of antibiotics resistance genes in the sampling points was determined usingqPCR.
2
Selected genes from microarray analysis were validated usingqPCR.
3
Expression of key genes involved in fatty acid oxidation and esterification were measured in placental tissue usingqPCR.
4
Tissue tropism and transgene expression were assessed usingqPCR and reverse-transcriptase qPCR following topical application of the vectors.
5
The copy numbers, determined usingqPCR, were significantly higher in definite PCP and probable PCP patients than in colonized patients.
6
Methods: Human liver biopsies were obtained from 34 obese patients and AKR1D1 mRNA expression levels were measured usingqPCR.
7
In a validation cohort, circulating levels of these 3 miRs were assessed usingqPCR in 234 patients with HFrEF.
8
We employed four miRNA databases to predict the target genes of miR-486-5p and verified retrieved genes usingqPCR and western blotting.
9
Methods: Expression levels of miR-4443 and miR-5195 were evaluated in 45 ovarian tumor and 45 ovarian non-tumor tissue samples paraffin embedded usingqPCR.