1
Using lac fusions, this operon was confirmed to be regulated by RirA in response to Fe availability.
2
As judged by lac reporter fusions, this apparently positive control by RirA was probably due to post-transcriptional effects, in at least some cases.
3
Close homologues of RirA exist in other rhizobia and in the pathogen Brucella; many other bacteria have deduced proteins with more limited sequence similarity.
4
I wanted to write on the card, "Rira bien qui viva le dernier"; but alas!
5
Mutations in rirA reduced growth rates in Fe-replete and -depleted medium, but did not appear to affect symbiotic N(2) fixation.