These results were confirmed by cellular fractionation and immunoblotting for both cytosolic and nuclear compartments.

This paper describes the application of cellular fractionation with LC-MS for profiling histones in the cytosol and upon chromatin.

Studies involving cellular fractionation and microarray profiling have previously identified functionally distinct mRNA populations specific to cellular organelles and architectural compartments.

The results illustrate the utility of cellular fractionation in conjunction with LC-MS for the analysis of histone H1 throughout the cell.

First, we show that linker histones enriched by cellular fractionation gives less nuclear contamination and higher histone content than when prepared by nuclei isolation.

Using the Ramos Burkitt's lymphoma cell line, cellular fractionation was shown to give less nuclear contamination and higher histone content than preparations by nuclei isolation.

Consistent with this finding, sub- cellular fractionation of neonatal rat brain showed that GSK-3beta2 is present in fractions enriched in neurites and growth cones.

Cellular fractionation data revealed that in iron-rich media wild-type SvpA was exclusively secreted to the culture supernatant.

Cellular fractionation was also done with the exponentially growing cells and proteins were prepared from each fraction.

Cellular fractionation and immunocytochemical studies showed increased localization of TERT in the nucleus of core-expressing cells as compared to controls.