The prl mutants are proposed to act by destabilizing the closed conformation of the translocation channel.

Therefore the open and the closed conformations of this flexible loop are virtually unaffected by crystal contacts.

The dome loop, however, assumes a closed conformation in the presence of metal ions, or at low pH.

Inhibition of membrane fusion by Munc18-1 requires a closed conformation of syntaxin 1.

However, they differ in their ability to adopt closed conformations and thus to regulate the assembly of SNARE complexes.

Residue Tyr-100 at the entrance of the tunnel adopts either an open or closed conformation in the crystal structure.

Both open time and closed time distributions were biexponential, indicating at least two open and two closed conformations of the channel.

MD simulations were done of both the open and the closed conformations, with either MgADP or MgATP at the active site.

The deep active-site cleft is lined by conserved residues and has been captured in open and closed conformations in two different crystal forms.

Characterization of Tlg2p mutants and truncations indicate that this binding site corresponds to a closed conformation of Tlg2p.

MD simulation of SLADP docked in the closed conformation gave a probe mobility comparable to that seen in the EPR spectrum of the MV·SLADP complex.

Furthermore, the Tlg2p N-peptide competes with the closed conformation for binding, suggesting a fundamental regulatory mechanism for SM-syntaxin interactions in SNARE assembly and membrane fusion.

Structural conversion from the open to the closed conformation of MAD2 is required for MAD2 to be incorporated into the MCC.

Furthermore, it was found that a5-C76S or a5-C221S 20SPT were more likely to be found with the gate in a closed conformation.

Only syntaxin 7 adopts a closed conformation, whereas in vti1b and syntaxin 8, the N-terminal domains do not interact with the adjacent SNARE motifs.