A phagemid system was developed for the monovalent display of combinatorialantibody Fab libraries on the surface of filamentous phage M13.
2
For more than a decade, phage displayed combinatorialantibody libraries have been used to generate and select a wide variety of antibodies.
3
Antibodies that are expressed in cells infected with a lentiviral combinatorialantibody library are selected directly for function rather than only for binding.
4
A method is presented that uses combinatorialantibody libraries to endow cells with new binding energy landscapes for the purpose of regulating their phenotypes.
5
We describe a method based on a phagemid vector with helper phage rescue for the construction and rapid analysis of combinatorialantibody Fab libraries.