Enzyme activity of the four lysozymes could be confirmedbygel zymograms and showed charge differences on native-PAGE.
2
A large number of sites contained a novel LANA binding motif (TCCAT)3 which was confirmedbygel shift analysis.
3
Furthermore, the molecular mass calculated from the gene sequence was confirmedbygel permeation chromatography of crude extracts followed by activity measurements.
4
The size difference between the two forms of FBP was confirmedbygel filtration of both the native and the N-glycanase-treated proteins.