Factors that control recruitment of theca cells from ovarian stromal-interstitial cells are important for early follicle development in the ovary.

Therefore, KL is proposed to promote early follicle development by inducing proliferation and organization of stromal stem cells around small follicles.

In summary, TSP-1 and -2 were coordinately expressed in the extravascular compartment of the ovary during early follicle development.

One hundred and fifty-four early follicles were isolated by enzymic digestion and mechanical disruption.

However, the growth and survival of individual early follicles in encapsulated 3D culture have not been reported.