Which subset of human DCs should be targeted for optimal activation of T cell immunity, however, remains unknown.

This is the first report describing a chimpanzee-derived recombinant Ad as a vector for transduction of human DCs.

Gene expression profiles of ASC or CatB deficient human DCs show marked overlap with downregulation of genes implicated in DC function.

These data demonstrate an important role for ASC and CatB in regulating function of human DCs with overlapping effects on gene expression.

The gene delivery by fiber-mutant Ad was more efficient than that by conventional Ad in both murine DC lines and normal human DCs (NHDC).