Angiography results demonstrated occlusion of the internal CA siphons bilaterally, with moyamoya vessels.

To test the involvement of different classes of internal Ca(2+) stores, we used a pharmacological approach.

Astrocytes were mechanically stimulated with a glass pipette, which reliably caused an increase in internal Ca(2+) levels and glutamate release into the extracellular space.

To address this issue, we cultured purified solitary cortical astrocytes and monitored changes in their internal Ca(2+) levels and glutamate release into the extracellular space.