Employing opticaldiffraction tomography, 3-D RI tomograms of live cells with GNPs are precisely measured for an extended period with sub-micrometer resolution.
2
The ultimate limit of the technique is set by the gradient of the probe's field, allowing for a resolution beyond the opticaldiffraction limit.
3
Opticaldiffraction analysis of decorated microtubules showed a strong layer-line at this spacing, confirming that one kinesin head binds per tubulin heterodimer.