The organoid cultures contained also some myofibroblasts with potential to promote intestinal stem cell survival.

Mechanism underlying these phenomena and their diversity among vertebrates can be studied in 3D organoid cultures.

Here, we used homogeneous human bud tip organoid cultures and identified SMAD signaling as a key regulator of the bud tip-to-airway transition.

We aimed to identify regulators of neonatal intestinal epithelial development by testing a small library of epigenetic modifier inhibitors in Paneth cell-skewed organoid cultures.

We tested 33 organoid cultures derived from 22 patients with HGSC for defects in homologous recombination (HR) and replication fork protection.