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The expression of two other genes was confirmed by rtPCR.
2
Among them, we measured by q-RTPCR the overexpression of 11 of 14 genes.
3
Both patients had positive RTPCR for SARS-COV-2 in the second round of testing but the first assay tested was negative.
4
In addition, mRNA expression of estrogen sensitive genes in the uteri of these rats was measured using real time rtPCR.
5
The same safety protocols and process will apply with these operating as drive or walk-through testing centres for the rtPCR Covid-19 Antigen testing.
1
Microarray results were confirmed by real-time reversetranscriptionpolymerasechainreaction.
2
Alternative mRNA splicing of fibronectin was studied by reversetranscriptionpolymerasechainreaction.
3
The results were further confirmed at mRNA level by reversetranscriptionpolymerasechainreaction.
4
Expression of the transporter gene MDR1 was performed by quantitative reversetranscriptionpolymerasechainreaction.
5
The fusions were confirmed by specific amplification of the genomic breakpoints using reversetranscriptionpolymerasechainreaction.
1
This might complement future efforts to standardize MDR1 detection methods using RT-PCR.
2
The use and validity of RT-PCR results as gold standard are discussed.
3
Results: The transfection efficiency was confirmed with real-time RT-PCR and Western blotting.
4
Only patients with a confirmatory SARS-CoV-2 RT-PCR were included in the study.
5
Significantly altered microRNAs were validated in independent sample sets via quantitative RT-PCR.
1
Methods: Nucleocapsid gene was obtained by reversetranscriptionandpolymerasechainreaction from a SARS patient and inserted into the vector pFastBacHTa expressing baculovirus.
2
Although modal lengths of RNA fragments were shorter, reversetranscriptionandpolymerasechainreaction was able to amplify amplicons in range of 300 bp.
Usage of reverse transcriptase polymerase chain reaction in English
1
Three transcripts of PHF14 in human cell lines were identified by reversetranscriptasepolymerasechainreaction.
2
Expression of nutrition-sensitive genes was explored by quantitative reversetranscriptasepolymerasechainreaction in rat prefrontal cortex.
3
We employed a multimarker reversetranscriptasepolymerasechainreaction assay that demonstrates improved sensitivity over a single-marker approach.
4
Independent validation of these findings by quantitative real-time reversetranscriptasepolymerasechainreaction did not confirm these effects.
5
Methods: UT receptor expression as protein and as mRNA were assessed by Western blot and reversetranscriptasepolymerasechainreaction.
6
Northern blot and reversetranscriptasepolymerasechainreaction analysis demonstrated that the two rice profilin cDNA are anther or pollen specific.
7
Furthermore, reversetranscriptasepolymerasechainreaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein.
8
Independent validation of these findings by quantitative real-time reversetranscriptasepolymerasechainreaction and Western blot did not confirm these regulatory effects.
9
A role for hematopoietic chimerism in the establishment of CTLA4-Ig-induced transplantation tolerance was investigated using reversetranscriptasepolymerasechainreaction analysis of recipient tissues.
10
The expression of fibrosis-related molecules from mouse embryonic fibroblasts and infarcted heart was measured by Western blot and quantitative reversetranscriptasepolymerasechainreaction.
11
RS was assessed in 690 formalin-fixed paraffin-embedded tumor samples with quantitative reversetranscriptasepolymerasechainreaction by using prespecified genes and a previously validated algorithm.
12
The gold standard for diagnosis of HIV-1 infection in infants under the age of 2 years is DNA or reversetranscriptasepolymerasechainreaction.
13
Reversetranscriptasepolymerasechainreaction analysis for pancreatic alpha-amylase mRNA revealed specific signals in the normal stomach.
14
Reversetranscriptasepolymerasechainreaction and sequencing showed that the splicing mutation creates a stop codon downstream of exon-3.
15
Reversetranscriptasepolymerasechainreaction showed up-regulation of collagen type II and down-regulation of collagen type I on the negatively charged hydrogels.
16
Reversetranscriptasepolymerasechainreaction and bioinformatics analyses were then used to characterise the mutation and determine its effect on the protein product.
Translations for reverse transcriptase polymerase chain reaction