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The expression of two other genes was confirmed by rtPCR.
2
Among them, we measured by q-RTPCR the overexpression of 11 of 14 genes.
3
Both patients had positive RTPCR for SARS-COV-2 in the second round of testing but the first assay tested was negative.
4
In addition, mRNA expression of estrogen sensitive genes in the uteri of these rats was measured using real time rtPCR.
5
The same safety protocols and process will apply with these operating as drive or walk-through testing centres for the rtPCR Covid-19 Antigen testing.
1
This might complement future efforts to standardize MDR1 detection methods using RT-PCR.
2
The use and validity of RT-PCR results as gold standard are discussed.
3
Results: The transfection efficiency was confirmed with real-time RT-PCR and Western blotting.
4
Only patients with a confirmatory SARS-CoV-2 RT-PCR were included in the study.
5
Significantly altered microRNAs were validated in independent sample sets via quantitative RT-PCR.
1
Three transcripts of PHF14 in human cell lines were identified by reversetranscriptasepolymerasechainreaction.
2
Expression of nutrition-sensitive genes was explored by quantitative reversetranscriptasepolymerasechainreaction in rat prefrontal cortex.
3
We employed a multimarker reversetranscriptasepolymerasechainreaction assay that demonstrates improved sensitivity over a single-marker approach.
4
Independent validation of these findings by quantitative real-time reversetranscriptasepolymerasechainreaction did not confirm these effects.
5
Methods: UT receptor expression as protein and as mRNA were assessed by Western blot and reversetranscriptasepolymerasechainreaction.
1
Methods: Nucleocapsid gene was obtained by reversetranscriptionandpolymerasechainreaction from a SARS patient and inserted into the vector pFastBacHTa expressing baculovirus.
2
Although modal lengths of RNA fragments were shorter, reversetranscriptionandpolymerasechainreaction was able to amplify amplicons in range of 300 bp.
Usage of reverse transcription polymerase chain reaction in English
1
Microarray results were confirmed by real-time reversetranscriptionpolymerasechainreaction.
2
Alternative mRNA splicing of fibronectin was studied by reversetranscriptionpolymerasechainreaction.
3
The results were further confirmed at mRNA level by reversetranscriptionpolymerasechainreaction.
4
Expression of the transporter gene MDR1 was performed by quantitative reversetranscriptionpolymerasechainreaction.
5
The fusions were confirmed by specific amplification of the genomic breakpoints using reversetranscriptionpolymerasechainreaction.
6
Molecular diagnostic assays based on quantitative reversetranscriptionpolymerasechainreaction are used in most clinical laboratories.
7
Expression of potassium ions family in coronary artery was detected through reversetranscriptionpolymerasechainreaction quantitatively.
8
Semi-quantitative reversetranscriptionpolymerasechainreaction revealed that the expression of procollagen type III mRNA was inhibited.
9
Quantitative real-time reversetranscriptionpolymerasechainreaction was used to validate the two selected altered microR NAs.
10
Quantitative real-time reversetranscriptionpolymerasechainreaction analysis was used to analyze the transcriptional changes accompanying this transition.
11
The MRP5 gene expression levels were assessed by quantitative reversetranscriptionpolymerasechainreaction or RNase protection assay.
12
Expression levels of special fusion gene transcripts were detected in these tumors by reversetranscriptionpolymerasechainreaction.
13
Expression levels of efflux pump genes and regulators were examined by quantitative real-time reversetranscriptionpolymerasechainreaction.
14
A real-time reversetranscriptionpolymerasechainreaction was performed for HRV and HEV detection using the RV16 kit.
15
Quantitative reversetranscriptionpolymerasechainreaction demonstrated that these different abnormalities were associated with increased levels of MYC mRNA.
16
Methods: Expressions of genes and proteins were determined by quantitative reversetranscriptionpolymerasechainreaction, Western blot, and immunohistochemistry.
Translations for reverse transcription polymerase chain reaction