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We have developed an algorithm to analyze secretoryvesicle distribution and docking in electron micrographs.
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Taking together, the algorithm facilitates and expands the unbiased and efficient analysis of secretoryvesicle distribution and docking.
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The analysis of secretoryvesicle distribution is usually performed manually, which is labour-intensive and subject to human bias and error.
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At present, the role of Mts and kinesin in secretoryvesicle transport in exocrine epithelial cells has not been defined.
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Our results suggest that the molecular mechanism by which PTP-MEG2 promotes secretoryvesicle fusion involves the local release of NSF from a tyrosine-phosphorylated, inactive state.
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The Golgi apparatus was enlarged (80% after 10 days) with a higher ratio of secretoryvesicle to saccule volume densities.
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We expressed Rab3a mutants locked in the GTP- or GDP-bound form in wild-type and munc18-1 null mutant cells and analyzed secretoryvesicle distribution.
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Neurons secrete many diffusible signals from synaptic and other secretoryvesicles.
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However, in plant cells, there are obviously additional endosomal source compartments for secretoryvesicles.
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Secretoryvesicles dock at their target in preparation for fusion.
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It might be expected that the secretoryvesicles in different cell types use similar molecular machinery.
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The algorithm was highly accurate in recognizing secretoryvesicles and calculating their distribution including vesicle-docking analysis.
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Ciliated cells also are involved in the shedding of exosomes and secretoryvesicles in the lumen.
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The exocyst is a multisubunit complex required for vectorial targeting of a subset of secretoryvesicles.
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Unicellular root hairs elongate via localized tip growth, a process mediated by polar exocytosis of secretoryvesicles.
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Quantal release of the principal excitatory neurotransmitter glutamate requires a mechanism for its transport into secretoryvesicles.