1
The role of endogenous small RNAs in metazoan nuclei is largely unknown.
2
Their fragmentation generates some small RNAs that are highly resistant to degradation.
3
Dicer initiates RNA interference by generating small RNAs involved in various silencing pathways.
4
They are discovered either by sequencing small RNAs or computationally.
5
However, the mechanism by which these small RNAs inhibit translation has yet to be elucidated.
6
Bacterial small RNAs perform numerous regulatory roles, including acting as antitoxic components in toxin-antitoxin systems.
7
Small RNAs, generally expressed at low levels, are difficult to reach usable levels from limited material.
8
Thirty-one loci resided in or near regions transcribing small RNAs, thus suggesting a role for post-transcriptional regulation.
9
This result suggests that not all sequences identified as miRNAs belong in this category of small RNAs.
10
Many organisms, including parasitic nematodes, secrete small RNAs into the extracellular environment, largely encapsulated within small vesicles.
11
We independently verified the expression of these small RNAs from 5' end of mature tRNA using qRT-PCR.
12
In plants and fungi, small RNAs silence gene expression in the nucleus by establishing repressive chromatin states.
13
Small RNAs constitute a new and unanticipated layer of gene regulation present in the three domains of life.
14
The methods used by our laboratories to design capture probes and label enriched small RNAs are thoroughly described here.
15
The synthesis of highly structured small RNAs containing nonstandard nucleotides is of high interest for structural and functional investigations.
16
MicroRNAs are a class of small RNAs that do not code for proteins and yet function as gene regulators.
