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1 Total protein extracts were prepared and subjected to SDS gel electrophoresis.
2 Each protein preparation was subjected to SDS - PAGE followed by Western blot analysis with the antibodies.
3 Samples of left ventricle were extracted subjected to SDS - PAGE , and Western analyzed for PKC epsilon and PKM epsilon.
4 A band of 32P-labelled protein was coincident with the enzyme band when immunoprecipitates were subjected to SDS - PAGE and autoradiography.
5 Proteins were also prepared from rat salivary glands and subjected to SDS - PAGE , followed by Western blotting analysis with the PP1 gamma 1 antibody.
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