Assays were performed after diethyl ether liquid-liquid extraction from 250-microL plasma samples.

The method involves deproteinization with acetone followed by addition of butanol and diethyl ether.

Although CYP2E1 metabolizes diethyl ether and was previously suggested to be involved

All animals were surface-cooled to 20 degrees C (rectal temperature) under halothane- diethyl ether azeotrope anesthesia and divided into 4 equal groups.

Diethyl ether was an old-fashioned anesthetic, used to induce unconsciousness.