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Supernatant VEGF levels were measured using a flt-1 receptor capture ELISA.

At earlier stages, the blood islands of flt-1lcz homozygotes were abnormal, with angioblasts in the interior as well as on the periphery.

Although melanoma cells expressed both, neuropilin-1 and flt-1, exogenous homodimeric placenta growth factor had no effect on melanoma cell growth.

These results suggest that hypoxia-exposed Hep G2 cells not only produce VEGF but also evolve paracrine induction of flt-1 through VEGF-independent mechanisms.

Cytogenetics and FLT-3 internal tandem duplication did not predict EFS or OS.