This property makes minicircle DNA an excellent vector for non-viral gene therapy.

This method shows potential for non-viral gene delivery both in vitro and in vivo.

Stem cells are poorly permissive to non-viral gene transfection reagents.

Increasing the potential for exogenously added pDNA to bind intracellular transport cofactors may enhance the potency of non-viral gene transfer.

Anderson said the ovarian cancer study is just one demonstration of the potential uses for nanoparticles in non-viral gene therapy.

The elongated transfection period and relatively high levels of reporter gene expression are significant advantages over other non-viral gene therapy techniques.

We conclude that the Genosphere assembly methodology offers advantages for the development of effective, scalable and targetable non-viral gene delivery vectors.

In vivo electroporation-mediated gene therapy in large animals is gaining ground as one of the most important means for non-viral gene therapy.

Import of exogenous plasmid DNA (pDNA) into mammalian cell nuclei represents a key intracellular obstacle to efficient non-viral gene delivery.

VP22 fusion may enhance the efficiency of non-viral gene delivery when combined with the appropriate therapeutic transgene, target tissue and transfection method.

The understanding of tissue and cellular barriers is a prerequisite for the development of more efficient non-viral gene therapy protocols for CF patients.

Ultrasound application in the presence of microbubbles has shown great potential for non-viral gene transfection via transient disruption of cell membrane (sonoporation).

The combination of transposon-based non-viral gene transfer with the latest improvements of non-viral delivery techniques could provide a long-term therapeutic effect without compromising biosafety.

Aim: To develop a novel non-viral gene delivery system, which has a small particle size and a high transfection efficiency to hepatocyte and hepatoma cells.