A substance capable of bringing about the reduction of another substance as it itself is oxidized; used in photography to lessen the density of a negative or print by oxidizing some of the loose silver.
NADPH also serves as a reductant, but exhibits a much higher Km.
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The first and third phases are dependent on reductant concentration, whereas the second phase is not.
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Oxidation stops at about the same level of reduction that was reached by the corresponding reductant.
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The enzyme has a unique nucleotide specificity, with NADH being twice as effective as NADPH as a reductant.
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H(2)S is a strong reductant which can react with reactive oxygen species like superoxide anion and hydrogen peroxide.
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The position of this end point is sensitive to the potential of the reactants but is relatively insensitive to excess concentrations of oxidant or reductant.
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In this report, we aimed to increase ADO-mediated alkane production by converting an unused by-product, formate, to a reductant that can be used by ADO.
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We explored the hypothesis that this difference in cellular responsiveness results from antagonism of the reductant action of acetaminophen on the PGHSs by cellular peroxides.
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This methodology is fast and does not need chemical reductants, which would have an important impact in high-throughput proteomics research.
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NADH and ubiquinol were used as reductants in separate experiments, and a full spectrum UV-visible stopped flow kinetic method was employed.