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Significados de reverse transcriptase polymerase chain reaction en inglés

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rt-pcr

Method in molecular biology; variant of polymerase chain reaction (PCR), is a technique commonly used in molecular biology to detect RNA expression.

rt-pcr
Sinónimos
Examples for "rtpcr"
Examples for "rtpcr"
1
The expression of two other genes was confirmed by rtPCR.
Pubmed
2
Among them, we measured by q-RTPCR the overexpression of 11 of 14 genes.
Pubmed
3
Both patients had positive RTPCR for SARS-COV-2 in the second round of testing but the first assay tested was negative.
Pubmed
4
In addition, mRNA expression of estrogen sensitive genes in the uteri of these rats was measured using real time rtPCR.
Pubmed
5
The same safety protocols and process will apply with these operating as drive or walk-through testing centres for the rtPCR Covid-19 Antigen testing.
The South African
1
Microarray results were confirmed by real-time reverse transcription polymerase chain reaction.
Pubmed
2
Alternative mRNA splicing of fibronectin was studied by reverse transcription polymerase chain reaction.
Pubmed
3
The results were further confirmed at mRNA level by reverse transcription polymerase chain reaction.
Pubmed
4
Expression of the transporter gene MDR1 was performed by quantitative reverse transcription polymerase chain reaction.
Pubmed
5
The fusions were confirmed by specific amplification of the genomic breakpoints using reverse transcription polymerase chain reaction.
Pubmed
1
This might complement future efforts to standardize MDR1 detection methods using RT-PCR.
Pubmed
2
The use and validity of RT-PCR results as gold standard are discussed.
Pubmed
3
Results: The transfection efficiency was confirmed with real-time RT-PCR and Western blotting.
Pubmed
4
Only patients with a confirmatory SARS-CoV-2 RT-PCR were included in the study.
Pubmed
5
Significantly altered microRNAs were validated in independent sample sets via quantitative RT-PCR.
Pubmed
1
Methods: Nucleocapsid gene was obtained by reverse transcription and polymerase chain reaction from a SARS patient and inserted into the vector pFastBacHTa expressing baculovirus.
Pubmed
2
Although modal lengths of RNA fragments were shorter, reverse transcription and polymerase chain reaction was able to amplify amplicons in range of 300 bp.
Pubmed
Uso de reverse transcriptase polymerase chain reaction en inglés
1
Three transcripts of PHF14 in human cell lines were identified by reverse transcriptase polymerase chain reaction.
Pubmed
2
Expression of nutrition-sensitive genes was explored by quantitative reverse transcriptase polymerase chain reaction in rat prefrontal cortex.
Pubmed
3
We employed a multimarker reverse transcriptase polymerase chain reaction assay that demonstrates improved sensitivity over a single-marker approach.
Pubmed
4
Independent validation of these findings by quantitative real-time reverse transcriptase polymerase chain reaction did not confirm these effects.
Pubmed
5
Methods: UT receptor expression as protein and as mRNA were assessed by Western blot and reverse transcriptase polymerase chain reaction.
Pubmed
6
Northern blot and reverse transcriptase polymerase chain reaction analysis demonstrated that the two rice profilin cDNA are anther or pollen specific.
Pubmed
7
Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein.
Pubmed
8
Independent validation of these findings by quantitative real-time reverse transcriptase polymerase chain reaction and Western blot did not confirm these regulatory effects.
Pubmed
9
A role for hematopoietic chimerism in the establishment of CTLA4-Ig-induced transplantation tolerance was investigated using reverse transcriptase polymerase chain reaction analysis of recipient tissues.
Pubmed
10
The expression of fibrosis-related molecules from mouse embryonic fibroblasts and infarcted heart was measured by Western blot and quantitative reverse transcriptase polymerase chain reaction.
Pubmed
11
RS was assessed in 690 formalin-fixed paraffin-embedded tumor samples with quantitative reverse transcriptase polymerase chain reaction by using prespecified genes and a previously validated algorithm.
Pubmed
12
The gold standard for diagnosis of HIV-1 infection in infants under the age of 2 years is DNA or reverse transcriptase polymerase chain reaction.
Pubmed
13
Reverse transcriptase polymerase chain reaction analysis for pancreatic alpha-amylase mRNA revealed specific signals in the normal stomach.
Pubmed
14
Reverse transcriptase polymerase chain reaction and sequencing showed that the splicing mutation creates a stop codon downstream of exon-3.
Pubmed
15
Reverse transcriptase polymerase chain reaction showed up-regulation of collagen type II and down-regulation of collagen type I on the negatively charged hydrogels.
Pubmed
16
Reverse transcriptase polymerase chain reaction and bioinformatics analyses were then used to characterise the mutation and determine its effect on the protein product.
Pubmed