We hypothesized that PKP2 may regulate focaladhesion dynamics and cell migration.
1
Background: FAK localization to focaladhesions is essential for its activation and function.
2
Assembly and modulation of focaladhesions during dynamic adhesive processes are poorly understood.
3
Vinculin was identified as a component of focaladhesions and adherens junctions nearly 40 years ago.
4
Stress fibers and focaladhesions are complex protein arrays that produce, transmit and sense mechanical tension.
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This results in a net increase in active Rho, and increased stress fibers and focaladhesions.
Uso de focal contact em inglês
1
Nonetheless, focalcontact proteins are central to mechanisms that regulate skin cell motility.
2
These results implicate the peptide segment nearest to the transmembrane region in focalcontact localization.
3
Interaction between individual FNIII domains and beta1 integrins mediates focal adhesion kinase phosphorylation and subsequent stress fiber and focalcontact formation.
4
Remarkably, both focalcontact and hemidesmosomal proteins appear involved in determining the speed and directional migration of epidermal cells by modulating several signal transduction pathways.
5
PKC delta EC contained significantly greater numbers of focalcontacts per cell.
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Two days after plating, the majority of the focalcontacts were placed on the oxide lines.
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We review the molecular components, functions, dynamics and cross-talk of hemidesmosomes and focalcontacts in keratinocytes.
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Moreover, the number of focalcontacts was enhanced by the increase of RGDC peptide densities grafted onto the material surface.
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Critical Issues: Although many publications are centered on focalcontacts, their existence in tissues such as the skin is controversial.
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In transfected COS-1 cells, punctin is deposited in the cell substratum in a punctate fashion and is excluded from focalcontacts.
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The longest truncated beta 1 subunit tested, which is only four amino acids shorter than the wild type, does localize to focalcontacts.
12
Small focalcontacts were also observed with tubulovesicular structures, likely to be endosomes, and with sparse multivesicular bodies and lysosomes found in our reconstructions.