Methodology: Thirty-seven staphylococci strains were evaluated by detecting the icaA gene.
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In this study we evaluated the presence of the icaA gene in strains of Staphylococcus spp.
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In four methicillin-resistant Staphylococcus aureus isolates, environmental activation of icaADBC did not always correlate with increased biofilm production.
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We evaluated the hypothesis that the ica operon and biofilm production are associated with CoNS disease in this setting.
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Changes of ica genes detected by PCR were confirmed by southern hybridization, and their nucleotides were analyzed by DNA sequencing.
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There were no significant differences in the presence of the ica operon or CRA positivity among the four groups of strains.
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The polysaccharide adhesion mechanism encoded by the ica operon is currently the best understood mediator of biofilm development, and represents an important virulence determinant.
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Deletion or substitution of these 5 nucleotides within the wild-type ica locus augmented transcription of the ica locus and induced the strong biofilm-producing phenotype.
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While ica gene of Staphylococcus epidermidis is known to undergo phase variation by insertion of IS256, the phenomenon in Staphylococcus aureus has not been evaluated.
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Sequence analysis revealed a 5-nucleotide deletion within the promoter region of the ica locus in MN8m compared with the sequence in the wild-type locus.
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Mobilised ICA representatives will work with local agencies, services and affected policyholders.
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Possible recanalization of the occluded ICA within the first month was evaluated.
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ICA declined to comment on a list of questions submitted by Reuters.
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Conversely, stent placement in the proximal ICA was associated with reperfusion success.
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The aneurysm was removed and the wall of the ICA was reconstructed.
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The NFT and Cine Lumiere also give discount tickets to ICA affiliates.