CFSs replicate late in S phase, are cell-type specific and nest in large genes.

Yet, the presence of Trp codons destabilized 3' ends of transcripts in operons or large genes.

By explicitly considering gene size, GONOME avoids a systematic bias toward GO terms linked to large genes.

We found that inducing low transcription with a weak promoter destabilized large genes, whereas stimulating their transcription with strong promoters alleviated instability.

Here we addressed the relationships between transcription, replication, and instability by manipulating the transcription of endogenous large genes in chicken and human cells.

Our results indicate that the SSCP analysis of amplified genomic DNA fragments can be successfully used to rapidly screen mutation containing exons in large genes.

Among other advantages, lipid-DNA complexes have the ability to transfer very large genes into cells, but their efficiency is much lower than that of viruses.