1
Effects of ligand concentration, pH, and nonspecific binding are also examined.
2
The biotinylated probe showed a high level of nonspecific binding in cell enzyme-linked assays.
3
The model indicates that the amount of nonspecific binding can be estimated from a simple rule.
4
Furthermore, it was not possible to reduce to negligible levels nonspecific binding of 99mTc to the antibody.
5
The CFE is based on assumptions of nonspecific binding with experimental use of very low drug concentrations.
6
The strength of this nonspecific binding is roughly estimated and found to be essential for the binding kinetics.
7
The saturable protein binding of disopyramide was described by a hyperbolic model including a specific binding site and additional nonspecific binding.
8
A novel feature is the provision to estimate the nonspecific binding component in the assays as a parameter in the model.
9
Two major artifacts in this type of assay, i.e., nonspecific binding events and optically overlapping receptors, can be detected and corrected during analysis.
10
Stringent washing of the beads prior to elution minimizes the contamination of the enriched material with unlabeled proteins through coimmunoprecipitation or nonspecific binding.
11
In astrocytoma cells, the nonspecific binding determined by 1 microM S-145 was observed in high percentage to total binding.
12
Replication protein A (RPA) stimulates DNA end resection by Exo1 by both preventing nonspecific binding of Exo1 to and preventing degradation of single-stranded DNA.
13
Alp7R also shows the typical nonspecific binding activity of a DNA-binding protein: Alp7R-GFP (green fluorescent protein) associates with the chromosomes of cells that lack alp7C.
14
The use of a detergent (0.1% Lubrol PX or Triton X-100) decreased nonspecific binding, increasing the proportion of specific binding to 70% of total binding.
15
Nonspecific binding was a large proportion of total binding in all cases.
16
Nonspecific binding is modeled by a short-range attractive surface potential.
