Methods: Wounds were created in confluent monolayers of the intestinal epithelial cell line of IEC-6.

In contrast, the expression of bFGF and IL-8 was not diminished in tumor cells growing on confluent monolayers of normal cells.

Moreover, cells were able to attach and spread on the surface of the samples, and gradually grow into nearly confluent monolayers.

KAY-2-41 had no noticeable toxicity on confluent monolayers, but a cytostatic effect was seen on growing cells.

This was achieved by allowing infected murine cells to settle onto confluent monolayers of non-murine cells in a straightforward infectious center assay.