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1 The effect of prolonged exposure to a hypertonic medium on human lymphocytes during mitogenic stimulation with phytohemagglutinin was investigated.
2 The clustering persisted in cells treated with hypertonic medium to disperse the clathrin lattices, as validated by co-immunoprecipitation experiments.
3 Both the expansion of the excystment vacuole and the emergence of ciliates occurred even when the cysts were immersed in hypertonic medium .
4 After 4 days of growth in a hypertonic medium , the plating efficiency of EUE cells was reduced and cell growth was significantly slowed.
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