A system for regulated heterologous gene expression in the filamentous fungus Penicillium chrysogenum was established.

Metabolic control analysis is used to identify the rate-limiting step in the penicillin biosynthetic pathway in Penicillium chrysogenum.

The biosynthesis of penicillin, a secondary metabolite produced by Penicillium chrysogenum, is subject to sophisticated genetic and metabolic regulation.

Continuous cultures of the penicillin producing fungus Penicillium chrysogenum have been analyzed with respect to the macromolecular composition of the mycelium.

Physiological effects of phenoxyacetic acid, the penicillin V side-chain precursor, on steady-state continuous cultures of Penicillium chrysogenum have been studied both theoretically and experimentally.