1
Results & discussion: The SSH library contained thousands of positive clones.
2
Sibling analysis revealed that the four antiserum- positive clones encoded three immunologically-distinct parasite antigens.
3
Then, the reactivity of normal and other leukemia sera with positive clones were performed.
4
One hundred and twenty-two dot blot positive clones from infected subtracted library were sequenced.
5
The positive clones were selected with G418 and confirmed by Western blot and indirect immunofluorescence labelling.
6
The reactivity of the antigen- positive clones with sera from humans infected with related parasites was also assessed.
7
Activity of the positive clones was measured by the nitro blue tetrazolium-reduction assay in the presence of cyanide.
8
The average size of inserts was about 420bp by analysis of 30 positive clones.
9
These antisera have been used to characterize the antigens in P. falciparum that correspond to the various antigen- positive clones.
10
The nucleotide and predicted amino acid sequences of the two positive clones were very similar to those of rat FcRn.
11
Results: 175 single cell clones were obtained by complex cloning, and 119 of those were positive clones.
12
Twenty-one strongly positive clones were found by hybridization of about 5000 bacterial colonies, nine of them with the inserts encoding ammodytoxin A.
13
Antisera raised by immunizing mice and rabbits with cloned antigens were used to characterize the P. falciparum proteins corresponding to the antigen- positive clones.
14
Two positive clones were sequenced to identify ORFs based on similarities to known proteins, and to ESTs using bioinformatics, and manually by a curator.
15
Most of the positive clones showed a 2- to 3-fold higher expression in PROb cells when compared with REGb cells.
16
In a search for protein kinase CK2 beta subunit binding proteins using the two-hybrid system, more than 1000 positive clones were isolated.
