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Both RRs are DNA-binding proteins that bind to promoter-proximal elements in the pln regulon.
2
We demonstrated that the plnABCD operon codes for an autoregulatory unit capable of activating its own promoter.
3
How phosphorylation regulates the activity of the pln response regulators was also accessed by direct mutagenesis at their phosphorylation sites.
4
This raises the question whether a functional peptide is being produced, even though RT-PCR studies showed that the plnE gene is obviously expressed.
5
Furthermore, heterologous expression of the plnABCD operon in a Lactobacillus sake strain showed that the conditioned growth medium contained the active induction factor.