In addition, the upregulation of protein expression was confirmed by immunoblot analysis.
2
Expression of the exogenous protein was assessed by immunoblot and immunohistochemistry analyses.
3
Positive patients had a similar yet completely unique immunoblot pattern of IgE binding.
4
Research on alternative testing strategies that eliminate the IgM immunoblot entirely is warranted.
5
One protein at approximately 100 kD was identified by immunoblot analysis as alpha-actinin.
1
Proteinimmunoblot analysis using antibodies specific for the NH(2) and COOH terminus of Sec61alpha was used to map the location of proteolysis cleavage sites.
1
Selected serum samples were retested by westernblotting and the results analysed.
2
We determined their expression patterns by relative real-time PCR and westernblotting.
3
Immunohistochemistry and westernblotting of PDAC tissues were compared with control tissues.
4
Cellular levels of apoptosis and autophagy-related proteins were measured by westernblotting.
5
Methods: p27 protein of MC was detected with westernblotting analysis.
1
The potential changes in protein expression were investigated using westernblot analysis.
2
This result was replicated in westernblot analyses from microdissected BNST samples.
3
Gene and protein expression levels were analyzed using RT-qPCR and westernblot.
4
Full-length and cleaved propeptide myostatin levels were quantified by westernblot analysis.
5
Spinal cord protein expression of both receptors was determined by westernblot.
6
To analyze the role of autophagy, westernblot and immunohistochemistry was performed.
7
Immunohistochemistry and westernblot localized and determined differential expression of P4 receptors.
8
Moreover, the protein abundance changes of CmACO are supported by westernblot experiments.
9
RT-qPCR and westernblot analysis were used to determine the BLACAT1 molecular mechanism.
10
Successful transfection was confirmed by using RT-PCR and westernblot analysis.
11
Methods: HDGF expression in the retinal was analyzed with westernblot and immunohistochemistry.
12
The results were then confirmed by histological and westernblot analysis.
13
RT-qPCR and westernblot were used to detect the expressional levels of genes.
14
Notch-1 nuclear expression was evaluated by immunofluorescence and westernblot analysis.
15
In addition, westernblot analysis showed decreases in active Notch and HEY1 proteins.
16
The expression levels of Bcl-2 and Bax were measured using westernblot analysis.