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1
Jiménez, how sophisticated is the methodology for growing cultures of humanES cells?
2
However, recent reports describe variability in epigenetic states in comparisons among different humanES cell lines.
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Here we show that this finding is also true with humanES cells (hESCs).
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The results show that epigenetic variants arise within humanES cell cultures after cell line derivation.
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Moreover, overexpression or suppression of human GPM6A in humanES cells led to an increase or decrease, respectively, of neuronal migration.
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In this manuscript we describe the properties of two humanES cell lines, BG01 and BG02.
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HumanES (hES) cell lines have only recently been generated, and differences between human and mouse ES cells have been identified.
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This system should permit identification of regulatory pathways for human cardiomyocyte proliferation and may facilitate expansion of cardiomyocytes from humanES cells for therapeutic purposes.
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These data provide a detailed characterization and an initial gene expression profile for the BG01 and BG02 humanES cell lines.