It is concluded that a hybridoma cell line stably secretes specific mAb against UGP2.
2
The monoclonal antibody was produced by hybridoma that was screened through cell fusion techniques.
3
In comparison, hybridoma cells are only adsorbed to the surface and do not spread at all.
4
The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.
5
A stable hybridoma cell line was established.
6
This approach allows for the rapid selection of monoclonal antibodies without the restraints of the conventional hybridoma approach.
7
The method was also employed to rescue IgG variable sequences and generate functional R-mAbs from a non-viable cryopreserved hybridoma.
8
All the eight kinds of positive hybridoma cells were mixed, cloned, screened by protein array, and definite dilution cloned.
9
We passively transfer these antibodies by intraperitoneal hybridoma implantation and by systemic administration of purified anti-ganglioside antibodies in mice.
10
The mice can be immunized; and human sequence, antigen specific, monoclonal antibodies can be obtained using conventional rodent hybridoma technology.
11
Human monoclonal antibodies (HuMAbs) were generated with hybridoma fusion methods using regional nodal lymphocytes of colon carcinoma patients.
12
Among these factors, the impact of micronutrients on the production of MAbs by mouse hybridoma cells has not fully been explored.
13
Inhibition of NF-kappaB activity in a T cell hybridoma leads to decreased FasL expression and apoptosis upon T cell receptor stimulation.
14
We now demonstrate that expression of hCD4 results in an equivalent stimulation of antigen responses by this mouse T cell hybridoma.
15
Blood-nerve barrier studies indicate that animals implanted with hybridoma had leaky blood-nerve barrier compared to mice that received systemically administered anti-ganglioside antibodies.
16
The hybridoma cell was adapted to serum-free medium and antibody was produced in a hollow fiber cell culture system (Technomouse).