Significats de hybridomas en anglès

In the present report, we have investigated the peculiar behavior of B-cell hybridomas with respect to IL-6 dependence.

We speculated that the loss of IL-6-dependent growth is due to the well-known chromosomal instability of B-cell hybridomas.

These results provide further evidence for an important role of LFA-1 in invasion and metastasis of mouse T-cell hybridomas.

The rat-mouse TT hybridomas thus generated were screened for antigen-dependent interleukin 2 production, for antigen-dependent polyclonal helper activity, and for surface phenotype.

Immune complexes cut out from immunoelectrophoresis gels and murine hybridomas were used as a source of specific anti-Antigen 5 and anti-Antigen B antibodies.

B cells could also present CII-derived peptides to antigen-specific DQ8-restricted hybridomas reinforcing the role of B cells in presentation of antigens to T cells.

With some hybridomas, however, we found a considerable degree of processing by populations of cultured LC at high doses of Ag or Ag-presenting cells.

Mouse, human and rat mast cells have been shown to express major histocompatibility complex II molecules and present antigens to specific T-cell hybridomas in vitro.

Comparison between T cell hybridomas that expressed full-length CD8 alpha and a deletion mutant lacking part of the cytoplasmic domain revealed identical responsiveness for H-2Kb.

We demonstrate that although newly generated hybridomas are IL-6 dependent, many hybridomas lose this dependency at frequencies as high as 50%, shortly after fusion.

This model implies the existence of an "IL-6 dependency" gene, the loss of which makes hybridomas capable of proliferating in the absence of IL-6.

It is concluded that a hybridoma cell line stably secretes specific mAb against UGP2.

The monoclonal antibody was produced by hybridoma that was screened through cell fusion techniques.

In comparison, hybridoma cells are only adsorbed to the surface and do not spread at all.

The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

A stable hybridoma cell line was established.