1 AFLP analysis allowed to distinguish 51 types and largely confirmed rep-typing results.
2 Here, we present a systematic approach for obtaining such information from AFLP markers.
3 This analysis was consistent with the AFLP analysis, although of much lower resolution.
4 The AFLP analysis technique produced some degree of discrimination among F. tularensis subsp.
5 The most promising AFLP marker was then selected for further characterization.
6 Individual AFLP genotyping of the marker on all selected animals confirmed a significant difference.
7 Thus, data from AFLP markers can be combined with microsatellite data for measuring genetic diversity.
8 Phylogenetic analysis of the fluorescent AFLP results reveals five genetic groups in these group 1 bacilli.
9 Seven primer combinations were used for AFLP analysis.
10 The microsatellite-enriched library was constructed using the fast isolation by AFLP of sequences containing repeats method.
11 The detailed protocol is presented with PCR results from a variable AFLP fragment from Bacillus anthracis.
12 In contrast, S. globosa exhibited consistent global distribution of identical AFLP types, suggesting another type of dispersal.
13 A reliable conversion procedure allowed five AFLP markers to be successfully converted into CAPS and SCAR markers.
14 Screening of this population with PCR, RFLP and AFLP markers identified several plants with crossovers near Mi.
15 In AFLP analysis, bacterial genomic DNA is digested with a restriction enzyme and ligated to adapter oligonucleotides.
16 The percent similarities of the AFLP profiles of this subspecies compared to those of F. tularensis subsp.
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