Objectives: Archaeological dentalcalculus is a rich source of host-associated biomolecules.
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Female students have significantly few gingivitis and dentalcalculus than male students in both groups.
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Importantly, however, dentalcalculus is more accurately described as a calcified microbial biofilm than a host tissue.
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Methods: A total of 93 artificial teeth coated with artificial dentalcalculus were examined in this study.
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Conclusions: The results of this study demonstrate the in vitro effectiveness of ADPJ in the removal of dentalcalculus, without causing damage to tooth surfaces.
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The percentage of gingivitis and dentalcalculus in students 11 to 13 years were 75.33% and 35.86%, respectively.
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All dentalcalculus samples exhibited damage patterns consistent with ancient DNA, and mitochondrial sequences were estimated to be 92-100% endogenous.
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Discussion: Dentalcalculus is a viable alternative source of human DNA that can be used to reconstruct full mitogenomes from archaeological remains.