Mammospheres with the characteristics of stem-like cells were collected by long-term serum- freesuspension cultivation.
2
This work suggests that hESCs can differentiate in simple serum- freesuspension cultures to produce the large number of cells required for transplantation studies.
3
These expanded CTCs exhibited different proportions of epithelial-mesenchymal phenotypes and were transferable for further expansion as spheroids in serum- freesuspension or 3D cultures.
4
Surviving TH+ neurons, derived from the cells differentiated in serum- freesuspension cultures, were detected 8 weeks after transplantation into 6-OHDA-lesioned rat brains.