Here we constructed a mutant murine P-gp with a shortened linker to facilitate structural determination.

For neutral species, a method that allowed the complete structural determination using mass spectrometry was used.

Here we report the NMR resonance assignments of SSO1118 for further structural determination and functional studies.

Particle extraction from experimental micrographs thus can be laborious and presents a major practical bottleneck in cryo-EM structural determination.

High-resolution structural determination and dynamic characterization of membrane proteins by nuclear magnetic resonance (NMR) require their isotopic labeling.

Bicelles can also be spun at the magic angle and lead to the 3D structural determination of molecules in membranes.

During the past 3 years, the semi-automated structural genomics pipeline has been set up from cloning, expression, purification, and ultimately to structural determination.

Volta Phase Plate (VPP) has become an invaluable tool for cryo-EM structural determination of small protein complexes by increasing image contrast.

The structural determination of the new toxin, including its absolute stereochemistry, has been performed by extensive NMR analysis and molecular mechanics and dynamics calculations.

Results: In this review, we discuss the challenges associated with the structural determination of parasite proteins including the difficulties in obtaining sufficient quantities of protein.

In addition, we examine some of the challenges remaining in structural determination, and what steps are underway to enhance our knowledge of these enigmatic proteins.

The recombinant ILT1 D1D2 domains behave as an equilibrium of both stable dimer and monomer in solution and yield ideal crystals for structural determination.

Structural determinations were carried out by chemical analysis and nuclear magnetic resonance spectroscopy.