Here we constructed a mutant murine P-gp with a shortened linker to facilitate structuraldetermination.
2
For neutral species, a method that allowed the complete structuraldetermination using mass spectrometry was used.
3
Here we report the NMR resonance assignments of SSO1118 for further structuraldetermination and functional studies.
4
Particle extraction from experimental micrographs thus can be laborious and presents a major practical bottleneck in cryo-EM structuraldetermination.
5
High-resolution structuraldetermination and dynamic characterization of membrane proteins by nuclear magnetic resonance (NMR) require their isotopic labeling.
6
Bicelles can also be spun at the magic angle and lead to the 3D structuraldetermination of molecules in membranes.
7
During the past 3 years, the semi-automated structural genomics pipeline has been set up from cloning, expression, purification, and ultimately to structuraldetermination.
8
Volta Phase Plate (VPP) has become an invaluable tool for cryo-EM structuraldetermination of small protein complexes by increasing image contrast.
9
The structuraldetermination of the new toxin, including its absolute stereochemistry, has been performed by extensive NMR analysis and molecular mechanics and dynamics calculations.
10
Results: In this review, we discuss the challenges associated with the structuraldetermination of parasite proteins including the difficulties in obtaining sufficient quantities of protein.
11
In addition, we examine some of the challenges remaining in structuraldetermination, and what steps are underway to enhance our knowledge of these enigmatic proteins.
12
The recombinant ILT1 D1D2 domains behave as an equilibrium of both stable dimer and monomer in solution and yield ideal crystals for structuraldetermination.
13
Structuraldeterminations were carried out by chemical analysis and nuclear magnetic resonance spectroscopy.