In organ culture KL significantly increased the number of thecacell layers around primary follicles.
2
Collagen IV was abundant in the thecacell compartment with low-level expression in the stroma and granulosa cells.
3
Fibronectin staining in the stroma and thecacell compartment increased throughout follicle development, while staining in the granulosa cell compartment decreased.
4
In addition, there is likely a primary thecacell defect that accounts for the majority of excess androgen production in this disorder.
5
Laminin was localized primarily to the thecacell compartment, with a defined ring at the exterior of the follicular granulosa cells marking the basement membrane.
6
The results in this study suggest that granulosa cell-derived KL appears to promote the formation of thecacell layers around small (i.e., primary) ovarian follicles.
7
Factors that control recruitment of thecacells from ovarian stromal-interstitial cells are important for early follicle development in the ovary.
8
During recruitment, thecacells organize into distinct layers around early developing follicles and establish essential cell-cell interactions with granulosa cells.
9
Recruitment of thecacells from ovarian stromal stem cells is proposed to involve cellular proliferation, as well as induction of theca cell-specific functional markers.